高级职称
首页 >> 师资队伍 >> 高级职称 >> Johannes Liesche
 

Johannes Liesche

作者:    来源:     发布日期:2016-01-05     浏览次数:

     

General information

Johannes Liesche, PhD, Associate Professor, Doctoral Supervisor. 2004-2008 German diploma at Humboldt University Berlin and University of Edinburgh. 2009-2012 PhD studies at University of Copenhagen and Cornell University.2012-2015 Postdoctoral studies at University of Copenhagen and New Zealand Institute for Plant and Food Research.

Research interests

Finding ways to increase plant productivity and yield, especially under changing climate conditions, is one of the top priorities for plant biologists. The transport of carbon from the leaves, where it is fixed, to roots, fruits and other organs that depend on carbon import for growth and development, is one potential target that has received relatively little attention so far. The carbon transport system is very complex, strictly regulated and interlinked with many other processes. My research aims at identifying the genetic factors that regulate the distribution of carbon in plants and discovering how carbon allocation is linked to environmental factors and stress responses.

Intercellular transport of carbohydrates can happen via two different pathways: i) active protein-mediated transport across the plasma membrane, ii) passive movement through cell wall channels. Over the last years, I have developed quantitative approaches based on live-cell microscopy and radiography, to measure both, the diffusive transport of carbohydrates through cell wall channels and active trans-membrane transport. In current projects, I am applying these methods in combination with theoretical modeling, metabolite analysis and the various techniques of molecular biology to investigate carbohydrate transport regulation at the cellular and molecular level.

 

Achievements in scientific research

Peer-reviewed papers in international journals:

Liesche J. How regulation of phloem transport could link potassium fertilization to increased growth. Tree Physiol. 2015.

Liesche J, Windt C, Bohr T, Schulz A, Jensen KH. Slower phloem transport in gymnosperm trees can be attributed to higher sieve element resistance. Tree Physiol. 2015 35(4).

Liesche J, Marek M, Günther-Pomorski T. Cell wall staining with Trypan Blue enables quantitative analysis of morphological changes in yeast cells. Front. Microbiol. 2015 (6)107.

Ronellenfitsch H, Liesche J, Jensen KH, Holbrook N, Schulz A, Katifori E. () Scaling of phloem structure and optimality of photoassimilate transport in conifer needles. Proc. R. Soc. B. 2015 (282)20141863.

Dölger J, Rademaker H, Liesche J, Schulz A, Bohr T. Diffusion and bulk flow in phloem loading - a theoretical analysis of the polymer trap mechanism. Physical Review E. 2014 (90)042704.

Liesche J, Ziomkiewicz I, Schulz A. Super-resolution imaging with Pontamine Fast Scarlet 4BS enables direct visualization of cellulose orientation and cell connection architecture in onion epidermis cells. BMC Plant Biol. 2013 (13)226.

Liesche J, Schulz A. Modeling the parameters for plasmodesmal sugar filtering in active symplasmic phloem loaders. Front. Plant Sci. 2013 (4)204.

Liesche J, Jensen K, Minchin PE, Bohr T, Schulz A. Theoretical and experimental determination of phloem translocation speeds in gymnosperm and angiosperm trees. Acta Hort. 2013 (991).

Chincinska I, Gier K, Krügel U, Liesche J, He H, Grimm B, Harren FJ, Cristescu S, Kühn C. Photoperiodic regulation of the sucrose transporter StSUT4 affects the expression of circadian-regulated genes and ethylene production. Front. Plant Sci. 2013 (4)26.

Linnik O, Liesche J, Tilsner J, Oparka K. Unravelling the structure of viral replication complexes at super-resolution. Front. Plant Sci. 2013 (4)6.

Liesche J, Schulz A. In vivo quantification of cell coupling in plants with different phloem loading strategies. Plant Physiol. 2012 (159)1.

Liesche J, Schulz A. Quantification of plant cell coupling with three-dimensional photoactivation microscopy. J Microsc. 2012 (247)1.

Liesche J#, Jensen KH#, Bohr T, Schulz A. Universality of phloem transport in seed plants. Plant Cell Environ, 2012 (35)6 (# shared first author).

Liesche J, Krügel U, He H, Chincinska IA, Hackel A, Kühn C. Sucrose transporter regulation at the transcriptional, post-transcriptional and post-translational level. J Plant Physiol. 2011 (168)12.

Bohr T, Jensen KH, Friis S, Liesche J, Schulz A. Universality of osmotically driven sap-flow in plants. Bull. Am. Phys. Soc. (56).

Liesche J, Martens HJ, Schulz A. Symplasmic transport and phloem loading in gymnosperm leaves. Protoplasma 2011 (248)1.

Liesche J, He H, Grimm B, Schulz A, Kühn C. Recycling of Solanum sucrose transporters expressed in yeast, tobacco, and in mature phloem sieve elements. Mol. Plant 2010 (3)6.

Liesche J, Schulz A, Krügel U, Grimm B, Kühn C. Dimerization and endocytosis of the sucrose transporter StSUT1 in mature sieve elements. Plant Signal Behav. 2008 (3)12.

Krügel U, Veenhoff LM, Langbein J, Wiederhold E, Liesche J, Friedrich T, Grimm B, Martinoia E, Poolman B, Kühn C. Transport and sorting of the Solanum tuberosum sucrose transporter SUT1 is affected by posttranslational modification. Plant Cell 2008 (20).

Chincinska IA, Liesche J, Krügel  U, Michalska J, Geigenberger P, Grimm B, Kühn C. Sucrose Transporter StSUT4 from potato affects flowering, tuberization, and shade avoidance response. Plant Physiol. 2008 (146).

 

Scholarly book chapters

Liesche J. Analysis of intended learning outcomes and evaluation of teaching activities in practical courses on microscopy. In Christiansen F (ed.) Improving University Science Teaching and Learning. (8)1-2. University of Copenhagen Press, Copenhagen, 2016.

Liesche J, Schulz A. Quantification of plant cell coupling with live-cell microscopy. In Heinlein M (ed.) Methods in Molecular Biology. Plasmodesmata: Methods and Protocols. Humana Press, Springer, New York, 2015.

Liesche J, Schulz A. Symplasmic transport in phloem loading and unloading. In Sowiński P, Sokołowska K (eds.) Symplastic transport in vascular plants. Springer, New York, 2013.

 

Contact details

Office No. 118

Email: liesche@nwsuaf.edu.cn

版权所有:  西北农林科技大学生命学院    地址:陕西杨凌农业高新技术产业示范区西农路22号  邮编:712100

主管领导:徐海            技术支持:艾特网络